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After the chlorophyll content of the lotus leaf was determined by the chlorophyll analyzer, the extraction process of the chlorophyll from the lotus leaf was even more justified. According to the extraction conditions of lotus leaf chlorophyll, the optimum extraction process of lotus leaf chlorophyll was that 85% acetone was used as the extractant, the ratio of solid to liquid was 1:8, extraction temperature was 500C, extraction time was 1.5h. Chlorophyll absorbance value of 2.2490 under optimized process
The stability of chlorophyll in lotus leaves was determined by chlorophyll analyzer. The results showed that illumination affected the stability of chlorophyll, and the chlorophyll abundance value decreased sharply when the illumination time was up to 3 h; the chlorophyll of lotus leaf had a certain high temperature resistance, which was 100°C and 70°C. The difference in chlorophyll absorbance after incubation is not significant; the pH value has a greater influence on the stability of the chlorophyll of the lotus leaf; when the pH value is 7 the absorbance value tends to be stable, that is, the chlorophyll of the lotus leaf is stable in a neutral or alkaline environment. H202 and ascorbic acid had obvious effects on the stability of chlorophyll of lotus leaf. Ascorbic acid had a stronger effect on the stability of chlorophyll; Zn2+, Cat+, Na+ had no obvious effect on the stability of chlorophyll, and A13+ and Fe3+ destroyed its stability. Cue+ has a very good effect on the color of chlorophyll. Therefore, in the process of extraction, processing, storage and use of lotus leaf chlorophyll, it should be carried out as far as possible under light, low temperature and alkaline conditions, and the adverse effects of H202, ascorbic acid, A13+, and Fe3+ should be prevented. It may be considered to prepare corresponding salts to improve their stability.
Chlorophyll analyzer provides relevant basis for extracting lotus leaf chlorophyll
Lotus root is an aquatic plant that does not require high growth conditions. As a common vegetable, it is distributed in the north and south of China, especially in the Yangtze River basin and the most southern provinces. The lotus leaf is the lotus leaf's leaf, its nutrient is rich, in addition to contains the carbohydrate, the fat, the protein and so on the composition, the flavonoid compound content is also very rich, but also contains the alkaloid. Chlorophyll as a natural pigment is widely used in medicine, food, cosmetics and other industries. With the improvement of people's living standards, the demand for natural pigments is also growing.