The principle of the determination of crude fiber content

The principle of the determination of crude fiber content

Vegetables and fruits contain a lot of crude fiber. Crude fiber is a major component of plant cell walls, including cellulose, hemicellulose, lignin, and keratin. Crude fiber cannot be digested and absorbed by the human body, but it is a very important "non-nutrient" substance. It can prevent certain diseases such as coronary heart disease, diabetes, colon cancer, constipation and other diseases and is a good helper for human health.

Since crude fiber is so important to the human body, it is necessary to determine the content of crude fiber in the plant. At this point, our first thought was to use laboratory methods to determine the content of crude fiber in the material. Of course, there is a simpler method. Some companies have developed instruments for measuring crude fiber based on laboratory measurements. They are simpler and more effective than laboratory methods. Such as crude fiber tester, CXC-06 fiber tester and other instruments, are able to determine the content of crude fiber in the material.

The focus of this article is not to use crude fiber analyzers and other instruments to determine the crude fiber content in sugar cane, but to introduce the laboratory's instrument and principle of determination of crude fiber content.

The principle of the determination of crude fiber content:

Samples were co-boiled with hot dilute acid and dilute alkali, and were separated by filtration, washed with residues, and then dried and ashed. The acid can be removed by hydrolysis of sugar, starch, pectin and part of the hemicellulose. Alkali can remove proteins, partially hemicellulose, lignin, and saponify fatty acids. It is treated with ethanol and ether. The residue obtained after drying is the crude fiber content minus the ash weight.

Crude fiber content determination instruments and reagents:

Instruments: Analytical balances, tissue mashers, crushers, electric plates, reflux devices, linens, Buchner hoppers, short-neck hoppers, filter bottles, crucibles, drying ovens, muffles, dryers; Reagents: Reagent purity All are analytically pure, the water used is distilled water or water of equivalent purity; sulfuric acid solution (c(1/2H2SO4,)=0.255±0.005mol/L); sodium hydroxide solution (c(NaOH)=0.313±0.005mol/L );95% Ethanol; Anhydrous ethyl ether; Defoamer; Asbestos